Key Documents

View All Documentation

C8571

Cathepsin B from human liver

Name: Cathepsin B from human liver
Brand: SIGMA

buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

Synonym(s):

Cathepsin B₁

Select a Size

Change View

About This Item

CAS Number:

9047-22-7

UNSPSC Code:

12352200

NACRES:

NA.32

EC Number:

3.4.22.1 (BRENDA, IUBMB)

MDL number:

MFCD00130748

Specific activity:

≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

Biological source:

human liver

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

biological source

human liver

Quality Level

200

form

buffered aqueous solution

specific activity

UniProt accession no.

P07858

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSB(1508)

General description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Application

Cathepsin B from human liver has been used:

as a positive control in the screening Cathepsin B inhibitor assay
in wall extension (expansin activity) assay
in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Packaging

Package size based on protein content

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Other Notes

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

高风险级别生物产品--人源产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis

Canbay A, et al.

The Journal of Clinical Investigation, 112(2), 152-159 (2003)

The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.

Musil DJ, et al.

The Embo Journal, 10(9), 2321-2330 (1991)

Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis

Gu J, et al.

Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)

Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways

Wu X, et al.

Hepatology, 47(6), 1905-1915 (2008)

Grass group I pollen allergens (beta-expansins) lack proteinase activity and do not cause wall loosening via proteolysis

Li LC and Cosgrove DJ

European Journal of Biochemistry, 268(15), 4217-4226 (2001)

View All Related Papers

Articles

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

组织蛋白酶B的酶活检测

组织蛋白酶B是具有广泛特异性的溶酶体半胱氨酸蛋白酶。本方案采用Nα–CBZ–Arg–Arg–7–酰胺基-4-甲基香豆素作为荧光检测组织蛋白酶B活性的底物。

Global Trade Item Number

SKU	GTIN
C8571-25UG	04061833522509

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service