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Merck
CN

C9210

Cyanogen bromide-activated Agarose

lyophilized powder

Synonym(s):

Agarose, cyclic carbonimidate, CNBr-Activated Agarose

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About This Item

CAS Number:
68987-32-6
UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00163385

Key Documents

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Product Name

Cyanogen bromide-activated Agarose, lyophilized powder

form

lyophilized powder

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 2.5-4.5 mL

capacity

≥10 mg/mL binding capacity (BSA)(via amino groups)

suitability

suitable for chromatography

storage temp.

−20°C

Quality Level

200

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Application

Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

Physical form

Lyophilized powder stabilized with lactose

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000512947
0000512947
0000426609
0000426609
0000393638

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?

    The control for this product is Product No. CL4B200 (Sepharose CL-4B).

  4. What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?

    Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare.  In addition, C9210 is a cross-linked resin and C9142 is not.

  5. When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?

    The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Supported Planar Bilayers for Study of the Immunological Synapse.
Dustin, M.L., et al.
Current Protocols in Immunology, 76, 18-18 (2007)
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
Martiniano M Ricardi et al.
BMC plant biology, 14, 29-29 (2014-01-16)
Identifying the target genes of transcription factors is important for unraveling regulatory networks in all types of organisms. Our interest was precisely to uncover the spectrum of loci regulated by a widespread plant transcription factor involved in physiological adaptation to
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