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C9616

Anti-Cytochrome c antibody produced in sheep

Name: Anti-Cytochrome c antibody produced in sheep
Brand: SIGMA

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CYC

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About This Item

UNSPSC Code:

12352203

MDL number:

MFCD01864291

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IHC (p), WB

Citations:

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Properties

biological source

sheep

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 15 kDa

species reactivity

rabbit, rat, human, canine

concentration

~0.5 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using human heart tissue, indirect immunofluorescence: 5-10 μg/mL using human MCF-7 cells, western blot: 0.1-0.2 μg/mL using whole extracts of MCF−7, Jurkat, Rat−1, MDCK cells and extract of rat kidney or rat heart

UniProt accession no.

P99999

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CYCS(54205)
rat ... Cycs(25309)

Description

General description

Anti-Cytochrome c produced in sheep using purified rabbit cytochrome c conjugated to keyhole limpet hemocyanin (KLH).

Cytochrome c is an electron transport protein released from mitochondria as an early committed event in apoptosis. Cytochrome c and dATP are cofactors for the mammalian apoptosome, which is composed of Apaf-1, Bcl-2, and procaspase 9. When caspase 9 is activated, activation of other caspases follow including the death protease caspase 3. The release of cytochrome c is inhibited by the presence of Bcl-2 on these organelles preventing the initiation of apoptosis. In cells induced by several apoptotic agents (such as UV irradiation, staurosporine, and overexpression of Bax), caspase inhibitors do not prevent cytochrome c release.

Immunogen

rabbit cytochrome c.

Application

Anti-Cytochrome c antibody produced in sheep has been used in:

immunocytochemistry
immunostaining
immunoblotting
immunohistochemistry

Biochem/physiol Actions

Cytochrome c controls cellular electron transport and energy metabolism. It is involved in the transfer of electrons between complex III and complex IV in the mitochondrial electron transport chain. It is involved in apoptosis and activates the death protease caspase-3 (CCP32). The presence of Bcl-2 on the organelles inhibits the release of cytochrome c from mitochondria during apoptosis. Along with Apaf-1, and procaspase-9, it forms an essential component of vertebrate ”apoptosome”. Activation of caspase-9 and other capsases directs apoptosis. Serum cytochrome c is a sensitive apoptotic marker in vivo, and increased serum cytochrome c level can serve as a negative prognostic marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c