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Merck
CN

C9994

CAMK2β, active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

CAM2, CAMKB, MG C29528

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
Specific activity:
4820-6521 nmol/min·mg
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

4820-6521 nmol/min·mg

mol wt

~58 kDa

UniProt accession no.

Q13554

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... CAMK2B(816)

Biochem/physiol Actions

CAMK2β belongs to the serine/threonine protein kinase family and to the type II multifunctional Ca2+/calmodulin-dependent protein kinase subfamily. CAMK2β showed wide tissue and cell distribution, and one of CAMK2β variant predominated in adult brain. The ratio of CAMK2α and CAMK2β protein levels were inversely related during activity in hippocampal neurons. CAMK2β is a prominent kinase in the central nervous system and may function in long-term potentiation and neurotransmitter release.

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCR1304
SLBC5564V

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Tara C Thiagarajan et al.
Neuron, 36(6), 1103-1114 (2002-12-24)
We show that alpha and betaCaMKII are inversely regulated by activity in hippocampal neurons in culture: the alpha/beta ratio shifts toward alpha during increased activity and beta during decreased activity. The swing in ratio is approximately 5-fold and may help
R M Tombes et al.
Biochimica et biophysica acta, 1355(3), 281-292 (1997-03-01)
CaMK-II (the (type II) multifunctional Ca2+/CaM-dependent protein kinase) has been implicated in diverse neuronal and non-neuronal functions, including cell growth control. CaMKII expression was evaluated in a variety of human tumor cell lines using RT-PCR (reverse transcriptase coupled polymerase chain

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