OX-89

Con A activated mouse spleen

The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

Mouse NS1 x rat

This antibody recognises the ligand for OX40 (CD134 ligand) on dendritic cells and some activated lymphocytes.

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Seed at 5 x 10,000 cells/ml. Subculture when cells reach 500,000 - 1,000,000 cells/ml (usually after 3-4 days), 5% CO₂, 37° C. Start in 20% FBS when recovering from frozen.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.