RaBE (Slow Growth)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
rat lung
packaging
tube of 5 μg 04100602-DNA-5UG
pkg of vial of cells 04100602-1VL
growth mode
Adherent
karyotype
Hypotetraploid, n=74 chromosomes (median)
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Rat Bronchial Epithelial Cells (RaBE) Slow Growth
Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. This is a slower growing derivative of the cell line RaBE, Sigma Catalogue number. 04100601, adapted to culture medium without growth factors.
Preparation Note
Ham?s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding at 20,000 cells/25cm2 with 5ml medium or 5x100,000 cells/75cm2 with 20ml, using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture once a week, double volume at medium change
Other Notes
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