biological source
rat lung
packaging
tube of 5 μg 04100602-DNA-5UG, pkg of vial of cells 04100602-1VL
growth mode
Adherent
karyotype
Hypotetraploid, n=74 chromosomes (median)
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Rat Bronchial Epithelial Cells (RaBE) Slow Growth
Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. This is a slower growing derivative of the cell line RaBE, Sigma Catalogue number. 04100601, adapted to culture medium without growth factors.
Preparation Note
Ham?s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding at 20,000 cells/25cm2 with 5ml medium or 5x100,000 cells/75cm2 with 20ml, using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture once a week, double volume at medium change
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.