RaBE (Slow Growth)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
生物来源
rat lung
包装
tube of 5 μg 04100602-DNA-5UG
pkg of vial of cells 04100602-1VL
生长模式
Adherent
核型
Hypotetraploid, n=74 chromosomes (median)
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
生化/生理作用
Rat Bronchial Epithelial Cells (RaBE) Slow Growth
Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. This is a slower growing derivative of the cell line RaBE, Sigma Catalogue number. 04100601, adapted to culture medium without growth factors.
制备说明
Ham?s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding at 20,000 cells/25cm2 with 5ml medium or 5x100,000 cells/75cm2 with 20ml, using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture once a week, double volume at medium change
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
新产品
此项目有
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持