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About This Item
UNSPSC Code:
41106514
Biological source:
canine kidney
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
canine kidney
description
Canine Cocker Spaniel Kidney Sialic Acid Over Expression
packaging
tube of 5 μg 05071502-DNA-5UG, pkg of vial of cells 05071502-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Enhanced expression of 6-linked sialic acids
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Canine Cocker Spaniel Kidney Sialic Acid Over Expression
This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.
Preparation Note
DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Other Notes
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Regulatory Information
动植物源性产品
低风险生物材料
动植物来源培养基
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