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About This Item
UNSPSC Code:
41106514
Biological source:
human embryo
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Not specified
biological source
human embryo
packaging
tube of 5 μg 06011803-DNA-5UG, pkg of vial of cells 06011803-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A non seminomatous germ cell carcinoma derived cell line. This cell line was established from a single cell clone from a subline of cell line 2102Ep passaged through a nude mouse. This cell line is tumourigenic in nude mice.
Human embryonal carcinoma
Preparation Note
DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 10% CO2; 37°C.
Other Notes
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