C22 (Clara)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
mouse lung
packaging
tube of 5 μg 07021401-DNA-5UG
pkg of vial of cells 07021401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.
Mouse lung epithelial progenitor cell
Preparation Note
DMEM + 2 mM Glutamine + 4% Fetal Bovine Serum (FBS) + 8 supplements:
0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3
For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.
0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3
For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.
Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;10% CO2; 33°C. Doubling time 48h
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service