biological source
bovine connective tissue
packaging
tube of 5 μg 08052101-DNA-5UG, pkg of vial of cells 08052101-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A bovine (Bos taurus, Holstein-Fresian) articular cartilage progenitor cell line able to re-differentiate into articular chondrocytes after extensive population doublings (>30pd).
Bos taurus (Holstein-Fresian) articular cartilage progenitor
Preparation Note
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 50ÂÂμg/ml ascorbate + 10mM HEPES
Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.