biological source
bovine connective tissue
packaging
tube of 5 μg 08052101-DNA-5UG, pkg of vial of cells 08052101-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A bovine (Bos taurus, Holstein-Fresian) articular cartilage progenitor cell line able to re-differentiate into articular chondrocytes after extensive population doublings (>30pd).
Bos taurus (Holstein-Fresian) articular cartilage progenitor
Preparation Note
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 50ÂÂμg/ml ascorbate + 10mM HEPES
Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC.
Other Notes
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