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About This Item
UNSPSC Code:
41106514
Biological source:
mouse nerve
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal No of chromosomes is 86
Morphology:
Neuronal
biological source
mouse nerve
packaging
tube of 5 μg 08062515-DNA-5UG, pkg of vial of cells 08062515-1VL
growth mode
Adherent
karyotype
Modal No of chromosomes is 86
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Used in the generation of cell hybrids.
Biochem/physiol Actions
A 6-thioguanine-resistant clone of N4 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line lacks any appreciable activity of either choline acetyltransferase or tyrosine hydroxylase enzymes.
Mouse neuroblastoma
Preparation Note
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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