生物来源
mouse nerve
包装
tube of 5 μg 08062515-DNA-5UG
pkg of vial of cells 08062515-1VL
生长模式
Adherent
核型
Modal No of chromosomes is 86
形态学
Neuronal
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Used in the generation of cell hybrids.
生化/生理作用
A 6-thioguanine-resistant clone of N4 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line lacks any appreciable activity of either choline acetyltransferase or tyrosine hydroxylase enzymes.
Mouse neuroblastoma
制备说明
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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法规信息
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