MCC14/2 cell line human

Human Merkel carcinoma

Merkel cell carcinoma cell line. Cell Bank Australia reference number: CBA1340.

Optimal split ratio 1:8 (seeding density 10⁴ cells/cm²).Cells incubated at 37 °C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 15% FCS/FBS

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Helen Leonard - Queensland Institute of Medical Research, Australia

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.