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TKE2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 11033107, mouse cornea. Epithelial cells isolated from basal limbal layer.

Synonym(s):

TKE, TKE-2

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About This Item

UNSPSC Code:
41106514
Biological source:
eye, mouse (CD-1)
Growth mode:
Adherent
Karyotype:
Normal karyotype of the female mouse CD1 strain
Morphology:
Epithelial; small and regular cobblestone-like shape

Key Documents

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biological source

eye, mouse (CD-1)

packaging

tube of 5 μg 11033107-DNA-5UG, pkg of vial of cells 11033107-1VL

gender

female

growth mode

Adherent

karyotype

Normal karyotype of the female mouse CD1 strain

morphology

Epithelial; small and regular cobblestone-like shape

products

Stains positive for nuclear p63 expression and K14 keratin.

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

Related Categories

Biochem/physiol Actions

Mouse cornea epithelial progenitor cells
Not specified
TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

Preparation Note

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 102 - 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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