Skip to Content
Merck
CN

TKE2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 11033107, mouse cornea. Epithelial cells isolated from basal limbal layer.

Synonym(s):

TKE, TKE-2

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
41106514

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

eye
mouse (CD-1)

packaging

tube of 5 μg 11033107-DNA-5UG
pkg of vial of cells 11033107-1VL

gender

female

growth mode

Adherent

karyotype

Normal karyotype of the female mouse CD1 strain

morphology

Epithelial; small and regular cobblestone-like shape

products

Stains positive for nuclear p63 expression and K14 keratin.

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

Related Categories

Biochem/physiol Actions

Mouse cornea epithelial progenitor cells
Not specified
TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

Preparation Note

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 102 - 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service