TKE2
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 11033107, mouse cornea. Epithelial cells isolated from basal limbal layer.
别名:
TKE, TKE-2
生物来源
eye
mouse (CD-1)
包装
tube of 5 μg 11033107-DNA-5UG
pkg of vial of cells 11033107-1VL
性别
female
生长模式
Adherent
核型
Normal karyotype of the female mouse CD1 strain
形态学
Epithelial; small and regular cobblestone-like shape
产品
Stains positive for nuclear p63 expression and K14 keratin.
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
生化/生理作用
Mouse cornea epithelial progenitor cells
Not specified
TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.
制备说明
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 102 - 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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