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UNSPSC Code:
41106514
Biological source:
eye, mouse (CD-1)
Growth mode:
Adherent
Karyotype:
Normal karyotype of the female mouse CD1 strain
Morphology:
Epithelial; small and regular cobblestone-like shape
biological source
eye, mouse (CD-1)
packaging
tube of 5 μg 11033107-DNA-5UG, pkg of vial of cells 11033107-1VL
gender
female
growth mode
Adherent
karyotype
Normal karyotype of the female mouse CD1 strain
morphology
Epithelial; small and regular cobblestone-like shape
products
Stains positive for nuclear p63 expression and K14 keratin.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Biochem/physiol Actions
Mouse cornea epithelial progenitor cells
Not specified
TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 102 - 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.
Other Notes
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