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Merck
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LIM1899 cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 12062001, human colon, Epithelial; cells are small rounded and grow in clusters

Synonym(s):

LIM 1899, LIM-1899

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About This Item

UNSPSC Code:
41106514
Biological source:
human colon
Growth mode:
adherent
Morphology:
Epithelial; cells are small rounded and grow in clusters

Key Documents

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biological source

human colon

packaging

tube of 5 μg 12062001-DNA-5UG, pkg of vial of cells 12062001-1VL

growth mode

adherent

morphology

Epithelial; cells are small rounded and grow in clusters

shipped in

dry ice

storage temp.

−196°C

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Biochem/physiol Actions

Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.

Preparation Note

Optimal split ratio 1:8 (seeding density 1 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Regulatory Information

低风险生物材料
常规特殊物品
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