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About This Item
UNSPSC Code:
41106514
Biological source:
mouse mesothelium
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
biological source
mouse mesothelium
packaging
tube of 5 μg 12070506-DNA-5UG, pkg of vial of cells 12070506-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
receptors
Not specified
relevant disease(s)
cancer
Biochem/physiol Actions
AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. Cell Bank Australia reference number: CBA1374.
Mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA)
Not specified
Preparation Note
Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Cells will require passaging twice weekly at this seeding density.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
Regulatory Information
低风险生物材料
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