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F2442

胎牛血清

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

别名:

FBS, FCS, 血清

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关于此项目

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

胎牛血清(FBS)是一种由低、高分子量生物分子组成的复杂混合物,具有最佳的生长促进和抑制功能。FBS中含有如生长因子、蛋白质、维生素、微量元素、激素等多种分子。这些分子对于细胞的生长和维持至关重要。它是细胞培养基中的一种常见补剂。FBS来自于小牛血。它不含有纤维蛋白和凝血因子。FBS的热灭活可阻断补体系统。来源于凝结血的FBS是真核细胞,尤其是哺乳动物细胞培养中最广泛使用的未确定补充剂。它通常与在1950年代和1960年代开发的经典培养基一起使用。这些包括多种常用培养基,如Minimum Essential培养基(MEM)、Dulbecco′s改良Eagles培养基(DME,DMEM)、Iscove′s改良Eagles培养基(IMDM)、M199以及Roswell Park Memorial Institute培养基RPMI-1640。

Application

胎牛血清还可作为组分用于:
  • 血管周围巨噬细胞样黑素细胞(PVM/M)生长培养基
  • 合成输卵管液(SOF)
  • Eagle′s minimum essential培养基(EMEM)
  • neurobasal培养基
  • Hyclone McCoys 5 A培养基
  • Hanks培养基

Biochem/physiol Actions

FBS仍然是一种常用的培养基补充剂,因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子,并通过难以在无血清培养基(SFM)系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。

Analysis Note

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

Other Notes

对是否存在细菌、病毒、支原体、内毒素以及血红蛋白进行了检测。在无菌条件下用0.1微米膜过滤三次。

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)

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全球贸易项目编号

货号GTIN
F2442-100ML04061838338709
F2442-24X500ML04061838255372
F2442-1L04061837034589
F2442-50ML04061838255396
F2442-500ML04061838255389
F2442-6X500ML04061838255402

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