BHK21C13-2P
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84111301, hamster kidney, Not specified
biological source
hamster kidney
packaging
tube of 5 μg 84111301-DNA-5UG
pkg of vial of cells 84111301-1VL
growth mode
Suspension
karyotype
2n = 44, pseudodiploid
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus replication studies
Biochem/physiol Actions
Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.
Hamster Syrian kidney
Preparation Note
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37°C. Cells need to be grown in a techni-stirrer or they may start to re-attach.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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