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About This Item
UNSPSC Code:
41106514
Biological source:
mouse skin
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Lymphoblast
biological source
mouse skin
packaging
tube of 5 μg 85011417-DNA-5UG, pkg of vial of cells 85011417-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Fusion partner, hybridisation and tumourigenicity studies
Biochem/physiol Actions
Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas.
Mouse myeloma
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
动植物源性产品
动物来源培养基
低风险生物材料
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Articles
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.