biological source
mouse skin
packaging
tube of 5 μg 85011417-DNA-5UG, pkg of vial of cells 85011417-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Fusion partner, hybridisation and tumourigenicity studies
Biochem/physiol Actions
Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas.
Mouse myeloma
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
动植物源性产品
低风险生物材料
此项目有
商品
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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