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About This Item
UNSPSC Code:
41106514
Biological source:
hamster ovary
Growth mode:
Adherent
Karyotype:
2n = 22
Morphology:
Epithelial
biological source
hamster ovary
packaging
tube of 5 μg 85011441-DNA-5UG, pkg of vial of cells 85011441-1VL
growth mode
Adherent
karyotype
2n = 22
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Genetic studies
Biochem/physiol Actions
Hamster Chinese ovary
Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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