AFRC MAC 3
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii, 85102265
biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 85102265-DNA-5UG
pkg of vial of cells 85102265-1VL
ImmunoDon
AO rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Glycoprotein of Chlamydomonas reinhardii
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat AO spleen
The antibody recognises the glycoprotein 2BII and labels both the whole cell surface and also the flagella. Binding is lost after acid hydrolysis or de-glycosylation of 2BII.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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