biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 85102265-DNA-5UG, pkg of vial of cells 85102265-1VL
ImmunoDon
AO rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-Glycoprotein of Chlamydomonas reinhardii
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat AO spleen
The antibody recognises the glycoprotein 2BII and labels both the whole cell surface and also the flagella. Binding is lost after acid hydrolysis or de-glycosylation of 2BII.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.