H129-19.6.8
85103101, Reactivity: Anti-L3T4, mouse, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Mouse x Rat Heterohybridoma
packaging
tube of 5 μg 85103101-DNA-5UG
pkg of vial of cells 85103101-1VL
ImmunoDon
LOU/WS1 rat spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-L3T4, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
Lyt-1+2- anti-I-Ak cytolytic T lymphocytes
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen
The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Regulatory Information
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