Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
41106514
Biological source:
Mouse x Rat Heterohybridoma
Isotype:
IgG2a, kappa
Technique(s):
cell culture | mammalian: suitable
Myeloma:
P3X63Ag8.653
Reactivity:
Anti-L3T4, mouse
biological source
Mouse x Rat Heterohybridoma
packaging
tube of 5 μg 85103101-DNA-5UG, pkg of vial of cells 85103101-1VL
ImmunoDon
LOU/WS1 rat spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-L3T4, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
Lyt-1+2- anti-I-Ak cytolytic T lymphocytes
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen
The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Regulatory Information
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service