H129-19.6.8

Lyt-1+2- anti-I-Ak cytolytic T lymphocytes

Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen

The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.