biological source
Mouse x Rat Heterohybridoma
packaging
tube of 5 μg 85103101-DNA-5UG, pkg of vial of cells 85103101-1VL
ImmunoDon
LOU/WS1 rat spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-L3T4, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
Lyt-1+2- anti-I-Ak cytolytic T lymphocytes
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Rat LOU/WS1 spleen
The antibody recognises a monomorphic determinant on the L3T4 molecule (the murine equivalent of CD4).
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS). Immediately post resuscitation of the cells use media containing 20% FBS, once the culture is established the level of FBS can then be reduced to 10% FBS.
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.