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Merck
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MiCL1 (S+L-)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
Biological source:
mink lung
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 30
Morphology:
Fibroblast-like
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biological source

mink lung

packaging

tube of 5 μg 86020603-DNA-5UG, pkg of vial of cells 86020603-1VL

growth mode

Adherent

karyotype

2n = 30

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Biochem/physiol Actions

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.
Mink lung, Moloney murine sarcoma virus infected

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

低风险生物材料
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