AFRC MAC 65

Peribacteroid material followed by peribacteroid material depleted of bacterial lipopolysaccharide

Rat Y3Ag1.2.3 x Rat LOU/Iap spleen

The antibody directed against peribacteroid membrane from Rhizobium-induced root nodules of Pisum sativum. The antigen is also found in plasma membranes and Golgi bodies, but not in endoplasmic reticulum ER. The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.