biological source
human prostate (fetal)
packaging
tube of 5 μg 86031405-DNA-5UG
pkg of vial of cells 86031405-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: CMV and HSV
Biochem/physiol Actions
Derived from a 14-18 week old human foetus. The cells support growth of CMV and HSV. This cell line has a finite life span.
Human Caucasian foetal foreskin fibroblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 8,11
vWA: 16,17
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 8,11
vWA: 16,17
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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