EIII
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Human x Mouse Hybridoma hybrid
packaging
tube of 5 μg 86072804-DNA-5UG
pkg of vial of cells 86072804-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
pJ3.11, p79a, CFTR DNA markers
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Somatic cell hybrid mapping
Biochem/physiol Actions
Human x mouse hybrid
This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.
Preparation Note
DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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