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About This Item
UNSPSC Code:
41106514
Biological source:
Human x Mouse Hybridoma hybrid
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
biological source
Human x Mouse Hybridoma hybrid
packaging
tube of 5 μg 86072804-DNA-5UG, pkg of vial of cells 86072804-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
pJ3.11, p79a, CFTR DNA markers
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Somatic cell hybrid mapping
Biochem/physiol Actions
Human x mouse hybrid
This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.
Preparation Note
DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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