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About This Item
UNSPSC Code:
41106514
Biological source:
Human x Mouse Hybridoma unknown/unspecified
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast-like
biological source
Human x Mouse Hybridoma unknown/unspecified
packaging
tube of 5 μg 86080602-DNA-5UG, pkg of vial of cells 86080602-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Somatic cell hybrid mapping
Biochem/physiol Actions
Mouse / Human hybrid, SV-40 transformed
The cell line CL21 has been established by hybridisation of C57 BL mouse peritoneal macrophages with SV40 transformed human skin fibroblasts. The human cells were derived from a patient with Lesch-Nyhan syndrome and are deficient in hypoxanthine guanine phosphoribosyltransferase (HGPRT). They have been reported to contain the human chromosome 7 and could also contain subchromosomal fragments of chromosome 17.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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