LS180
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021202, human colon, Epithelial-like
biological source
human colon
packaging
tube of 5 μg 87021202-DNA-5UG
pkg of vial of cells 87021202-1VL
growth mode
Adherent
karyotype
Modal no. 45 (2n=46)
morphology
Epithelial-like
products
Carcinoembryonic antigen (CEA), Mucin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.
Human Caucasian colon adenocarcinoma
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 by scraping; 5% CO2; 37°C.
Other Notes
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