LS180
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021202, human colon, Epithelial-like
生物来源
human colon
包装
tube of 5 μg 87021202-DNA-5UG
pkg of vial of cells 87021202-1VL
生长模式
Adherent
核型
Modal no. 45 (2n=46)
形态学
Epithelial-like
产品
Carcinoembryonic antigen (CEA), Mucin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.
Human Caucasian colon adenocarcinoma
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 by scraping; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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