生物来源
human colon
包装
tube of 5 μg 87021202-DNA-5UG
pkg of vial of cells 87021202-1VL
生长模式
Adherent
核型
Modal no. 45 (2n=46)
形态学
Epithelial-like
产品
Carcinoembryonic antigen (CEA), Mucin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human Caucasian colon adenocarcinoma
细胞系描述
Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.
应用
Tumourigenicity studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 by scraping; 5% CO2; 37°C.
其他说明
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