biological source
mouse brain
packaging
tube of 5 μg 87021902-DNA-5UG
pkg of vial of cells 87021902-1VL
growth mode
Aggregates in suspension
karyotype
Diploid
morphology
Small round cells growing as large aggregates
products
Adrenocorticotropic hormone (ACTH)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.
Mouse LAF1 pituitary gland tumour
Preparation Note
Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.
Other Notes
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