biological source
mouse brain
packaging
tube of 5 μg 87021902-DNA-5UG, pkg of vial of cells 87021902-1VL
growth mode
Aggregates in suspension
karyotype
Diploid
morphology
Small round cells growing as large aggregates
products
Adrenocorticotropic hormone (ACTH)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.
Mouse LAF1 pituitary gland tumour
Preparation Note
Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.