biological source
mouse connective tissue
packaging
tube of 5 μg 87032401-DNA-5UG, pkg of vial of cells 87032401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Genetic and biochemical studies
Biochem/physiol Actions
Derived from NCTC clone 929 in serum-free medium. The cells were adapted from horse serum containing medium to serum-free over a period of one year.
Mouse C34/An connective tissue
Preparation Note
Medium 199 + 2mM Glutamine + 0.5% Bacto-peptone (Difco).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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