生物来源
mouse connective tissue
包装
tube of 5 μg 87032401-DNA-5UG
pkg of vial of cells 87032401-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse C34/An connective tissue
细胞系描述
Derived from NCTC clone 929 in serum-free medium. The cells were adapted from horse serum containing medium to serum-free over a period of one year.
应用
Genetic and biochemical studies
培养基
Medium 199 + 2mM Glutamine + 0.5% Bacto-peptone (Difco).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C.
其他说明
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