biological source
mouse connective tissue
packaging
tube of 5 μg 87032401-DNA-5UG, pkg of vial of cells 87032401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
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Application
Genetic and biochemical studies
Biochem/physiol Actions
Derived from NCTC clone 929 in serum-free medium. The cells were adapted from horse serum containing medium to serum-free over a period of one year.
Mouse C34/An connective tissue
Preparation Note
Medium 199 + 2mM Glutamine + 0.5% Bacto-peptone (Difco).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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