CV-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
African green monkey kidney
packaging
tube of 5 μg 87032605-DNA-5UG
pkg of vial of cells 87032605-1VL
growth mode
Adherent
karyotype
2n = 60, pseudodiploid
morphology
Fibroblast
receptors
Not specified
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Biochem/physiol Actions
Monkey African Green kidney fibroblast
Used in Rous sarcoma virus (RSV) transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African green monkey kidney. Cells grow rapidly and form monolayers of fibroblast-like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37 °C.
Other Notes
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