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About This Item
UNSPSC Code:
41106514
Biological source:
rat kidney
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial-like
biological source
rat kidney
packaging
tube of 5 μg 87061001-DNA-5UG, pkg of vial of cells 87061001-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Growth factors
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Transformation, growth factors and virus studies: Kirsten MSV
Biochem/physiol Actions
Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.
Normal rat kidney, Kirsten MSV transformed
Preparation Note
DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
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