EBTr
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
bovine trachea
packaging
tube of 5 μg 87090202-DNA-5UG
pkg of vial of cells 87090202-1VL
growth mode
Adherent
karyotype
2n = 60
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: BVDV, IBRV and parainfluenza type 3 virus.
Biochem/physiol Actions
Bovine foetal trachea
Derived from the trachea of a male bovine foetus. The cell line can be used for isolation, replication and quantitation of BVDV, IBRV and parainfluenza type 3 virus. Growth and morphological characteristics decline beyond passage number 55.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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