Grunt Fin (GF)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
fish fin
packaging
tube of 5 μg 88010601-DNA-5UG
pkg of vial of cells 88010601-1VL
growth mode
Adherent
karyotype
Modal no. 47
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.
Fish Grunt fin tissue blue striped
Preparation Note
EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO3 + 20% FBS (Heat Inactivated)+ 58mM NaCl
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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