biological source
rat pancreas
packaging
tube of 5 μg 88031605-DNA-5UG
pkg of vial of cells 88031605-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Insulin and glucagon
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon.
Not specified
Rat NEDH islet tumour
Preparation Note
DMEM + 2 mM Glutamine + 5-10% Fetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Passage every 3-4 days.
Other Notes
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