biological source
mouse blood
packaging
tube of 5 μg 88041905-DNA-5UG, pkg of vial of cells 88041905-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation and virus studies: MuLV
Biochem/physiol Actions
Established from a lymphoma produced in an NFS/N mouse by injecting CaS-NS-7 ecotropic MuLV. Cells show pre-B cell markers and appear to be at a very early stage of B-cell differentiation.
Mouse Pro-B lymphoblast
Preparation Note
DMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50μM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.