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EA human cell line

88052080, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., IHW Number: 9081

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About This Item

UNSPSC Code:
41106514
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biological source

human blood

packaging

tube of 5 μg 88052080-DNA-5UG
pkg of vial of cells 88052080-1VL

ethnicity

Scandinavian

gender

male

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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This Item
EMU050031EMU082051EMU188561
MISSION® esiRNA targeting mouse Xirp1

EMU189871

MISSION® esiRNA

MISSION® esiRNA targeting mouse Xbp1

EMU050031

MISSION® esiRNA

MISSION® esiRNA targeting mouse Xdh

EMU082051

MISSION® esiRNA

MISSION® esiRNA targeting mouse Xirp2

EMU188561

MISSION® esiRNA

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

Gene Information

mouse ... XIRP1(22437), Xirp1(22437)

Gene Information

mouse ... XBP1(22433), Xbp1(22433)

Gene Information

mouse ... XDH(22436), Xdh(22436)

Gene Information

mouse ... XIRP2(241431), Gm1322(383709)

Ensembl | mouse accession no.

ENSMUSG00000079243

Ensembl | mouse accession no.

ENSMUSG00000020484

Ensembl | mouse accession no.

ENSMUSG00000024066

Ensembl | mouse accession no.

ENSMUSG00000075314

esiRNA cDNA target sequence

GCCTCCTACAAGTCTCTCCTCCCTGCACAGGATCCTCCTCTCCAACTTTCATCTCAGTCCAATCAGCTACAAAGAAGCTTCCGGAAGCTTCTAGCCCTCAGGGAAGCCATTATATCTCAGGGAAAAATACACACCTAGGTCAGGACATAGGTCAAGCCCTGCTCTACCAGAGAGATATCCAGGATCAGGCTGGGACAAAGGAGATGTGCATCGAGGGCGCTGTGCTCACAGGGCAGCCAAAGAATGTTCTGGAATTCCAGACTGGGTCAACTACCTCCAAGTCCTATGGAGCCATGAGAACAGTGACCGAGCAGTATGAGGAGATGGACCAATTTGGGAATACAGTCCTCACTTCCTCCACCACCATCACCCAGCACGCAGACCCACTGACGGACCCCAGACCCCAGCTCTGCCTGCACACTTCTCCCATGCTAAGGCAGTTACTCCACAGCCCCTCCAGGCTCAACAGTGACCTGGCAGAAGCTGAGATAACATGGACTCCCTGTAACAACTTCC

esiRNA cDNA target sequence

TTCCTTACCAGCCTCCCTTTCTCTGTCAGTGGGGACCTCATCAGCCAAGCTGGAAGCCATTAATGAACTCATTCGTTTTGACCATGTATACACCAAGCCTCTAGTTTTAGAGATCCCCTCTGAGACAGAGAGTCAAACTAACGTGGTAGTGAAAATTGAGGAAGCACCTCTAAGCTCTTCAGAAGAGGATCACCCTGAATTCATTGTCTCAGTGAAGAAAGAGCCTTTGGAAGATGACTTCATCCCAGAGCTGGGCATCTCAAACCTGCTTTCATCCAGCCATTGTCTGAGACCACCTTCTTGCCTGCTGGACGCTCACAGTGACTGTGGATATGAGGGCTCCCCTTCTCCCTTCAGTGACATGTCTTCTCCACTTGGTACAGACCACTCCTGGGAGGATACTTTTGCCAATGAACTTTTCCCCCAGCT

esiRNA cDNA target sequence

TGGAAGGCATAGGAAACACCAAGAAGCTGCATCCTGTCCAGGAGAGAATTGCCAAAAGCCATGGTTCCCAGTGTGGGTTCTGTACCCCTGGTATTGTCATGAGTATGTACACACTGCTCCGAAACAAGCCTGAGCCTACTGTCGAGGAGATCGAGAATGCCTTCCAAGGAAACCTCTGTCGCTGTACAGGCTATAGACCCATCCTCCAGGGATTCCGGACCTTTGCCAAGGATGGTGGGTGCTGTGGAGGAAGTGGAAACAACCCAAACTGCTGCATGAGCCAAACAAAGGACCAGACGATTGCTCCCTCATCTTCTTTATTCAACCCGGAGGATTTCAAACCTTTAGATCCCACCCAAGAGCCCATCTTTCCCCCAGAGTTGCTGAGGCTGAAAGACACTCCCCGGAAGACGTTGCGTTTTGAAGGGGAACGTGTGACCTGGATCCAGGTTTCAACCATGGAGGAACTGCTTG

esiRNA cDNA target sequence

CAGAAGGGGTCACTGAGTCGCCTGAGGCAGAAATGGGAATCTAGTGACTATCACAGAAATGAGAGCTATCCTGGAGGCAGCCGGTGCAAGCTCTTCCAGCTTAAGGAAAGCAACTTGCTTGAACCTGAAGGAGAGGCACTTTCAACACTTGACCCTCCAGAGGCACCAAGTCTGCCTTGCTGCAAAAAGGAAGAGGTATTGTGTGGAGAGCCTGAAGATGGAGTTCCTGAGGACCAGAATGACAAACTCAAAGACTGTGGACAGCCTGAAGTACTGAAGGAGGATTCCCTGACTGGCCGGCGCAGGATTGAACGCTTTTCCATTGCCCTAGATGAGCTGAGAAGTATATTTGAGTCTCCCAAGAGTAGCATAAACTCAGCTGGACCAGTTGAATATGTCCAAAAGGTAAGAAGCTATTTATTTCAGGATTACAGC

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

NCBI accession no.

NM_011724

NCBI accession no.

NM_013842

NCBI accession no.

NM_011723

NCBI accession no.

NM_001033477

General description

Consanguineous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 301
Cw: 702
B: 702
HLA DRA: 102
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 2
HLA DQA1: 102
HLA DQB1: 602
HLA DPA1: 1
HLA DPB1: 401

Serological Profile

HLA A: 3
HLA A ief: 3.2
HLA C: 7
HLA B: 7
HLA B ief: 7.2
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 4

Preparation Note

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Regulatory Information

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