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About This Item
UNSPSC Code:
41106514
Biological source:
Mouse x Rat Hybridoma nerve
Technique(s):
cell culture | mammalian: suitable
Morphology:
Neuronal
biological source
Mouse x Rat Hybridoma nerve
packaging
tube of 5 μg 88112302-DNA-5UG, pkg of vial of cells 88112302-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hybridisation, fusion partner and virus studies: Sendai virus
Biochem/physiol Actions
Mouse neuroblastoma x Rat glioma hybrid
Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm2 . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO2; 37°C. The cells do produce large amounts of CO2, media changes may be necessary.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
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Articles
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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