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Merck
CN

NG108-15

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88112302

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

Mouse x Rat Hybridoma nerve

packaging

tube of 5 μg 88112302-DNA-5UG
pkg of vial of cells 88112302-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Hybridisation, fusion partner and virus studies: Sendai virus

Biochem/physiol Actions

Mouse neuroblastoma x Rat glioma hybrid
Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm2 . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO2; 37°C. The cells do produce large amounts of CO2, media changes may be necessary.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Articles

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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