biological source
Mouse x Rat Hybridoma nerve
packaging
tube of 5 μg 88112302-DNA-5UG, pkg of vial of cells 88112302-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hybridisation, fusion partner and virus studies: Sendai virus
Biochem/physiol Actions
Mouse neuroblastoma x Rat glioma hybrid
Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm2 . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO2; 37°C. The cells do produce large amounts of CO2, media changes may be necessary.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
此项目有
商品
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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