NG108-15

Hybridisation, fusion partner and virus studies: Sendai virus

Mouse neuroblastoma x Rat glioma hybrid

Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm² . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO₂; 37°C. The cells do produce large amounts of CO₂, media changes may be necessary.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.