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UNSPSC Code:
41106514
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biological source

human skin

description

Human malignant melanoma

packaging

tube of 5 μg 88113005-DNA-5UG
pkg of vial of cells 88113005-1VL

growth mode

Adherent

karyotype

Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.
Tumourigenicity studies

Biochem/physiol Actions

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.
Human malignant melanoma

Preparation Note

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Other Notes

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