生物来源
human skin
描述
Human malignant melanoma
包装
tube of 5 μg 88113005-DNA-5UG
pkg of vial of cells 88113005-1VL
生长模式
Adherent
核型
Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.
Tumourigenicity studies
生化/生理作用
Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.
Human malignant melanoma
制备说明
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
其他说明
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